Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

Abstract Background People with diabetes mellitus, especially insulin-dependent diabetic patients, are a risk group for staphylococcal infections. Asymptomatic infection Staphylococcus aureus is common and favors dissemination of the microorganism, rendering these individuals source infection. This study aimed to characterize resistance profile, clonal profile sequence type, as well analyze prevalence factors nasal oropharyngeal carriage methicillin-susceptible (MSSA) methicillin-resistant S. (MRSA) isolated from in city Botucatu, SP, Brazil. Methods was collected nasopharynx oropharynx 312 community-dwelling over period 3 years (October 2015 December 2018). The isolates were characterized by susceptibility profiling, detection mec A gene, SCC typing, molecular typing PFGE MLST. associated MRSA determined logistic regression analysis. Results overall colonization 30.4% 4.8%, respectively. Fifteen 112 carried gene; type IV identified 10 isolates, I three, II two. Among 15 resistant (MRSA), four susceptible oxacillin/cefoxitin disc diffusion method one MSSA isolate sulfamethoxazole/trimethoprim. analysis revealed protective effect age lung disease, while lower-extremity ulcers factor . For MRSA, only male gender significantly multivariate Clonal demonstrated formation clusters among different identification ST5-IV, ST5-I, ST8-IV. Isolates carrying ST398 (ST398-IV). Conclusion Our findings reinforce importance epidemiological studies carriage, populations at high infections such diabetics. data suggest widespread population patients studied, emergence important lineages individuals.